Antigenic variation in the influenza A virus nonstructural protein, NS1
Identifieur interne : 002602 ( Main/Exploration ); précédent : 002601; suivant : 002603Antigenic variation in the influenza A virus nonstructural protein, NS1
Auteurs : Lorena E. Brown [États-Unis] ; V. S. Hinshaw [États-Unis] ; R. G. Webster [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1983.
English descriptors
- Teeft :
- Allantoic fluid, Antibody, Antigenic, Antigenic analysis, Antigenic determinants, Antigenic drift, Antigenic profile, Antigenic reactivity, Antigenic region, Antigenic regions, Antigenic relationships, Antigenic sites, Antigenic structure, Antigenic variation, Antigenically, Antigenicity, Ascitic, Ascitic fluid, Ascitic fluid preparations, Avian, Avian origin, Avian strains, Cell lysates, Compans, Competition experiments, Cytoplasmic, Cytoplasmic extracts, Cytoplasmic inclusions, Different patterns, Different strains, Form group, Human strains, Inclusion, Influenza, Influenza virus, Influenza viruses, Matrix, Matrix protein, Mdck cells, Molecular weight, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Other members, Overall correlation, Percentage inhibition, Point mutations, Protein, Sequence data, Spleen cells, Surface antigen subtype, Topographical analysis, Virus, Virus nucleoprotein.
Abstract
Abstract: The antigenic structure of the nonstructural (NS1) protein encoded by influenza type A virus was examined using monoclonal antibodies prepared against purified NSl inclusions isolated from the cytoplasm of infected cells. Topographical analysis by competitive radioimmunoassay indicated that three different overlapping antigenic regions were present on the NS1 of A/WSN/33 (HlNl). Immunoprecipitation studies using infected cell lysates showed that antigenic determinants on A/WSN/33 NSl are common to NSl proteins encoded by a wide range of viruses of human, swine, equine, and avian origin. Several avian strains, however, were found to encode antigenically variant NSl proteins which had either extensive changes in one or more antigenic regions or small changes in epitopes within a region suggestive of antigenic drift. There was no correlation between surface antigen subtype and the antigenic profile of the NSl protein. The antigenic relationships of NSl proteins shown in this study are in agreement with the available sequence data.
Url:
DOI: 10.1016/0042-6822(83)90123-X
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000A18
- to stream Istex, to step Curation: 000A18
- to stream Istex, to step Checkpoint: 001259
- to stream Main, to step Merge: 002735
- to stream Main, to step Curation: 002602
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Antigenic variation in the influenza A virus nonstructural protein, NS1</title><author><name sortKey="Brown, Lorena E" sort="Brown, Lorena E" uniqKey="Brown L" first="Lorena E." last="Brown">Lorena E. Brown</name></author><author><name sortKey="Hinshaw, V S" sort="Hinshaw, V S" uniqKey="Hinshaw V" first="V. S." last="Hinshaw">V. S. Hinshaw</name></author><author><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R. G." last="Webster">R. G. Webster</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:6226B07FA8175AFC6D25E74C75807C5A8C54C703</idno><date when="1983" year="1983">1983</date><idno type="doi">10.1016/0042-6822(83)90123-X</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-9XBMXRPW-3/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000A18</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000A18</idno><idno type="wicri:Area/Istex/Curation">000A18</idno><idno type="wicri:Area/Istex/Checkpoint">001259</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001259</idno><idno type="wicri:doubleKey">0042-6822:1983:Brown L:antigenic:variation:in</idno><idno type="wicri:Area/Main/Merge">002735</idno><idno type="wicri:Area/Main/Curation">002602</idno><idno type="wicri:Area/Main/Exploration">002602</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Antigenic variation in the influenza A virus nonstructural protein, NS1</title><author><name sortKey="Brown, Lorena E" sort="Brown, Lorena E" uniqKey="Brown L" first="Lorena E." last="Brown">Lorena E. Brown</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Virology and Molecular Biology, St Jude Children's Research Hospital, Memphis, Tennessee 38101</wicri:regionArea><wicri:noRegion>Tennessee 38101</wicri:noRegion></affiliation></author><author><name sortKey="Hinshaw, V S" sort="Hinshaw, V S" uniqKey="Hinshaw V" first="V. S." last="Hinshaw">V. S. Hinshaw</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Virology and Molecular Biology, St Jude Children's Research Hospital, Memphis, Tennessee 38101</wicri:regionArea><wicri:noRegion>Tennessee 38101</wicri:noRegion></affiliation></author><author><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R. G." last="Webster">R. G. Webster</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Virology and Molecular Biology, St Jude Children's Research Hospital, Memphis, Tennessee 38101</wicri:regionArea><wicri:noRegion>Tennessee 38101</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1983">1983</date><biblScope unit="volume">130</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="134">134</biblScope><biblScope unit="page" to="143">143</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Allantoic fluid</term><term>Antibody</term><term>Antigenic</term><term>Antigenic analysis</term><term>Antigenic determinants</term><term>Antigenic drift</term><term>Antigenic profile</term><term>Antigenic reactivity</term><term>Antigenic region</term><term>Antigenic regions</term><term>Antigenic relationships</term><term>Antigenic sites</term><term>Antigenic structure</term><term>Antigenic variation</term><term>Antigenically</term><term>Antigenicity</term><term>Ascitic</term><term>Ascitic fluid</term><term>Ascitic fluid preparations</term><term>Avian</term><term>Avian origin</term><term>Avian strains</term><term>Cell lysates</term><term>Compans</term><term>Competition experiments</term><term>Cytoplasmic</term><term>Cytoplasmic extracts</term><term>Cytoplasmic inclusions</term><term>Different patterns</term><term>Different strains</term><term>Form group</term><term>Human strains</term><term>Inclusion</term><term>Influenza</term><term>Influenza virus</term><term>Influenza viruses</term><term>Matrix</term><term>Matrix protein</term><term>Mdck cells</term><term>Molecular weight</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Monoclonal antibody</term><term>Other members</term><term>Overall correlation</term><term>Percentage inhibition</term><term>Point mutations</term><term>Protein</term><term>Sequence data</term><term>Spleen cells</term><term>Surface antigen subtype</term><term>Topographical analysis</term><term>Virus</term><term>Virus nucleoprotein</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The antigenic structure of the nonstructural (NS1) protein encoded by influenza type A virus was examined using monoclonal antibodies prepared against purified NSl inclusions isolated from the cytoplasm of infected cells. Topographical analysis by competitive radioimmunoassay indicated that three different overlapping antigenic regions were present on the NS1 of A/WSN/33 (HlNl). Immunoprecipitation studies using infected cell lysates showed that antigenic determinants on A/WSN/33 NSl are common to NSl proteins encoded by a wide range of viruses of human, swine, equine, and avian origin. Several avian strains, however, were found to encode antigenically variant NSl proteins which had either extensive changes in one or more antigenic regions or small changes in epitopes within a region suggestive of antigenic drift. There was no correlation between surface antigen subtype and the antigenic profile of the NSl protein. The antigenic relationships of NSl proteins shown in this study are in agreement with the available sequence data.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Brown, Lorena E" sort="Brown, Lorena E" uniqKey="Brown L" first="Lorena E." last="Brown">Lorena E. Brown</name></noRegion><name sortKey="Hinshaw, V S" sort="Hinshaw, V S" uniqKey="Hinshaw V" first="V. S." last="Hinshaw">V. S. Hinshaw</name><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R. G." last="Webster">R. G. Webster</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002602 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002602 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:6226B07FA8175AFC6D25E74C75807C5A8C54C703
|texte= Antigenic variation in the influenza A virus nonstructural protein, NS1
}}
| This area was generated with Dilib version V0.6.33. |  |